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Transposon-indsættelse Sekventering som et redskab til at belyse bakterielle kolonisering faktorer i en Burkholderia gladioli Symbiont af Lagria villosa Biller
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Transposon-indsættelse Sekventering som et redskab til at belyse bakterielle kolonisering faktorer i en Burkholderia gladioli Symbiont af Lagria villosa Biller

Article DOI: 10.3791/62843-v • 09:55 min • August 12th, 2021
August 12th, 2021 •
1, 1,2, 1,3
1Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, 2Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, 3Department of Plant and Environmental Sciences, Section for Organismal Biology, University of Copenhagen

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Dette er en tilpasset metode til at identificere kandidat insekt kolonisering faktorer i en Burkholderia gavnlig symbiont. Billeværten er inficeret med et tilfældigt mutantbibliotek genereret via transposon mutagenese, og bibliotekets kompleksitet efter kolonisering sammenlignes med en kontrol dyrket in vitro.

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Biologi Burkholderia Symbiont Host kolonisering Transposon sekventering Tn5 transposon Lagria villosa
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