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JoVE Journal Biochemistry
Vurdering af indsendeokondrial protein lokalisering i spirende gær saccharomyces cerevisiae
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Vurdering af indsendeokondrial protein lokalisering i spirende gær saccharomyces cerevisiae

Article DOI: 10.3791/62853-v • 08:55 min • July 19th, 2021
July 19th, 2021 •
1, 1, 1, 1
1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo

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På trods af de seneste fremskridt, mange gær mitokondrie proteiner stadig med deres funktioner helt ukendt. Denne protokol giver en enkel og pålidelig metode til at bestemme underkastelsesalisering af proteiner, som har været grundlæggende for udredningen af deres molekylære funktioner.

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Biokemi udgave 173 mitokondrier spirende gær Saccharomyces cerevisiae submitochondriale rum submitochondrialfraktionering underkastelses-lokalisering sonikering kulbonatudvinding western blot
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