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Immunology and Infection

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JoVE Journal Immunology and Infection
Aanpassing van gastro-intestinale organoïden voor pathogene infectie en single cell sequencing onder bioveiligheidsniveau 3 (BSL-3) omstandigheden
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Aanpassing van gastro-intestinale organoïden voor pathogene infectie en single cell sequencing onder bioveiligheidsniveau 3 (BSL-3) omstandigheden

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

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Dit protocol beschrijft hoe menselijke intestinale organoïden kunnen worden geïnfecteerd vanaf hun apicale of basolaterale kant om gastheer / pathogeeninteracties op eencellig niveau te karakteriseren met behulp van eencellige RNA-sequencing (scRNAseq) -technologie.

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Immunologie en infectie menselijke intestinale organoïden eencellige RNA-sequencing bioveiligheidsniveau 3 organoïde infectie enterische pathogenen apicale en basolaterale infectie
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