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Journal / Immunology and Infection / Anpassning av gastrointestinala organoider för…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Anpassning av gastrointestinala organoider för patogeninfektion och encellssekvensering under biosäkerhetsnivå 3 (BSL-3) tillstånd
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Journal (Immunology and Infection)
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Journal (Immunology and Infection)
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
 
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Anpassning av gastrointestinala organoider för patogeninfektion och encellssekvensering under biosäkerhetsnivå 3 (BSL-3) tillstånd

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

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Detta protokoll beskriver hur man infekterar mänskliga intestinala organoider från antingen deras apikala eller basolateral sida för att karakterisera värd/patogen interaktioner på encells nivå med hjälp av encelliga RNA sekvensering (scRNAseq) teknik.

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Immunologi och infektion nummer 175 humana tarmorganoider encellig RNA-sekvensering biosäkerhetsnivå 3 organoid infektion enteriska patogener apikal och basolateral infektion
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