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Cancer Research

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JoVE Journal Cancer Research
Analytische bepaling van mitochondriale functie van weggesneden vaste tumorhomogenaten
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Analytische bepaling van mitochondriale functie van weggesneden vaste tumorhomogenaten

Article DOI: 10.3791/62875-v • 11:32 min • August 6th, 2021
August 6th, 2021 •
1,2,3, 1, 2,4, 1,3
1Integrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism, Pennington Biomedical Research Center, 3Department of Nutrition, Case Western Reserve University, 4Department of Clinical Nutrition, University of Texas Southwestern Medical Center

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We ontwikkelden een praktisch protocol en een analytische benadering om mitochondriale oxidatieve fosforylering en elektronenoverdrachtscapaciteit in verse tumorhomogenaten te evalueren. Dit protocol kan eenvoudig worden aangepast om verschillende mitochondriale functies te onderzoeken die bijdragen aan het initiëren, progressie en behandelingsrespons van kanker.

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Kankeronderzoek Nummer 174
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