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Journal / Biology / 使用活性氧敏感荧光探针分析小鼠肠道类器官中的氧化应激…
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使用活性氧敏感荧光探针分析小鼠肠道类器官中的氧化应激
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使用活性氧敏感荧光探针分析小鼠肠道类器官中的氧化应激

Article DOI: 10.3791/62880-v • 09:31 min • September 17th, 2021
September 17th, 2021 •
1,3, 1,4, 1,2,5, 1,6
1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur

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本方案描述了一种使用定性成像和定量细胞术测定法检测肠道小鼠类器官中活性氧（ROS）的方法。这项工作可以潜在地扩展到其他荧光探针，以测试所选化合物对ROS的影响。

Tags

生物学，第175期，小鼠肠道类器官，ROS检测，流式细胞术分析，活体成像检测，肠干细胞，ROS敏感染料，氧化应激，荧光探针
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