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Analysera oxidativ stress i Murine Intestinala organoider med hjälp av reaktiva syre arter-känslig fluorogen sond
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Analysera oxidativ stress i Murine Intestinala organoider med hjälp av reaktiva syre arter-känslig fluorogen sond

Article DOI: 10.3791/62880-v • 09:31 min • September 17th, 2021
September 17th, 2021 •
1,3, 1,4, 1,2,5, 1,6
1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur

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Detta protokoll beskriver en metod för att upptäcka reaktiva syre arter (ROS) i intestinala murine organoider med kvalitativ avbildning och kvantitativa cytometri analyser. Detta arbete kan potentiellt utvidgas till andra fluorescerande sonder för att testa effekten av valda föreningar på ROS.

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Biologi nummer 175 Murine intestinala organoider ROS-detektion Flödescytometrianalys Live imaging detection intestinala stamceller ROS-känsligt färgämne oxidativ stress fluorogena sonder
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