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Neuroscience

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JoVE Journal Neuroscience
Fluoreszenz-aktiviertes Zellsortierungs-Radioliganden-behandeltes Gewebe (FACS-RTT) zur Bestimmung des zellulären Ursprungs des radioaktiven Signals
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Fluoreszenz-aktiviertes Zellsortierungs-Radioliganden-behandeltes Gewebe (FACS-RTT) zur Bestimmung des zellulären Ursprungs des radioaktiven Signals

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) ist ein leistungsfähiges Werkzeug, um die Rolle des 18-kDa-Translokatorproteins oder der Serotonin-5HT-2A-Rezeptorexpression bei der Alzheimer-Krankheit auf zellulärer Ebene zu untersuchen. Dieses Protokoll beschreibt die Ex-vivo-Anwendung von FACS-RTT im Rattenmodell TgF344-AD.

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Neuroscience Alzheimer's disease fluorescence-activated cell sorting to radioligand Treaded Tissues TgF344-AD Positronen-Emissions-Tomographie/Single Photon Emission Computed Tomography TSPO 5HT2AR
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