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Neuroscience

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JoVE Journal Neuroscience
Fluorescensaktivert cellesortering-radioligog behandlet vev (FACS-RTT) for å bestemme cellulær opprinnelse av radioaktivt signal
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Fluorescensaktivert cellesortering-radioligog behandlet vev (FACS-RTT) for å bestemme cellulær opprinnelse av radioaktivt signal

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Fluorescensaktivert cellesortering-radioligog behandlet vev (FACS-RTT) er et kraftig verktøy for å studere rollen til 18 kDa translokatorprotein eller Serotonin 5HT2A-reseptoruttrykk i Alzheimers sykdom i cellulær skala. Denne protokollen beskriver ex-vivo-applikasjonen av FACS-RTT i TgF344-AD-rottemodellen.

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Nevrovitenskap utgave 175 Alzheimers sykdom fluorescensaktivert cellesortering til radioligand tråkket vev TgF344-AD Positron-utslippstomografi / enkelt fotonutslipp beregnet tomografi TSPO 5HT2AR
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