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JoVE Journal Bioengineering
Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
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Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes

Article DOI: 10.3791/62889-v • 08:27 min • August 13th, 2021
August 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1
1Grup d’Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL)

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Summary
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Here, a simple protocol is presented for producing mRNA nanoparticles based on poly(beta aminoester) polymers, easy to be tailored by changing the encapsulated mRNA. The workflow for synthesizing the polymers, the nanoparticles, and their in vitro essential characterization are also described. A proof-of-concept regarding immunization is also added.

Tags

Synthesis Characterization MRNA-loaded Poly(Beta Aminoesters) Nanoparticles Vaccination Polymeric Nanoparticles Nucleic Acids Versatility Cell Types Particle Preparation Pipetting Lyophilization Laura Olmo Coral Garcia-Fernandez Maria Stampa Lopez-Pinto Maria Navalon-Lopez Marta Diaz-Caballero Polyplexes Formation Thaw Polymers C6 Peptide Sodium Acetate MRNA Milligrams Per Milliliter Vortex Genetic Material Solution Micro Centrifuge Tube Incubate Thermoblock Precipitate
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