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Journal / Biology / 从成年小鼠肾脏中分离细胞核以进行单核RNA测序…
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从成年小鼠肾脏中分离细胞核以进行单核RNA测序
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从成年小鼠肾脏中分离细胞核以进行单核RNA测序

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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在这里，我们提出了一种从冷冻小鼠肾脏中分离高质量细胞核的方案，该方案改善了髓质肾细胞类型的表征，并避免了酶组织解离引起的基因表达伪像。

Tags

生物学，第 175 期，肾脏，细胞核分离，转录组学，转录组测序，单核 RNA 测序，snRNA-seq
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