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Journal / Biology / Kerneisolering fra voksen musenyre til…
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Kerneisolering fra voksen musenyre til RNA-sekventering med en kerne
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Kerneisolering fra voksen musenyre til RNA-sekventering med en kerne

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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Her præsenterer vi en protokol til isolering af kerner af høj kvalitet fra frosne musenyrer, der forbedrer repræsentationen af medullære nyrecelletyper og undgår genekspressionsartefakter fra enzymatisk vævsdissociation.

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Biologi udgave 175 nyre kerneisolering transkriptomics transkriptomsekventering enkeltkerne RNA-sekventering snRNA-seq
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