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Aislamiento de núcleos de riñón de ratón adulto para secuenciación de ARN de un solo núcleo
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Aislamiento de núcleos de riñón de ratón adulto para secuenciación de ARN de un solo núcleo

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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Aquí, presentamos un protocolo para aislar núcleos de alta calidad de riñones de ratones congelados que mejoran la representación de los tipos de células renales medulares y evitan los artefactos de expresión génica de la disociación enzimática del tejido.

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Biología Número 175 riñón aislamiento de núcleos ómica secuenciación del transcriptoma secuenciación de ARN de un solo núcleo snRNA-seq
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