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Multiplekset fluorescerende immunhiistokjemisk farging av fire endometrie immuncelletyper i tilbakevendende abort
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Multiplekset fluorescerende immunhiistokjemisk farging av fire endometrie immuncelletyper i tilbakevendende abort

Article DOI: 10.3791/62931-v • 05:16 min • August 4th, 2021
August 4th, 2021 •
1, 1,2, 1, 1, 3, 1, 1,4, 1,5, 3, 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Til tross for fremskrittene i multiplex immunhiistokjemi og multispektral avbildning, karakteriserer tettheten og klyngen av store immunceller samtidig i endometrium fortsatt en utfordring. Dette dokumentet beskriver en detaljert multipleksfargingsprotokoll og bildebehandling for samtidig lokalisering av fire immuncelletyper i endometriumet.

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Biologi Utgave 174 Multipleks Fluorescens Immunceller Metoder Farging
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