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Coloração imunohistoquímica fluorescente multiplexada de quatro tipos de células imunes endometrial em aborto recorrente

Article DOI: 10.3791/62931-v • 05:16 min • August 4th, 2021
August 4th, 2021 •
1, 1,2, 1, 1, 3, 1, 1,4, 1,5, 3, 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Apesar dos avanços na imunohistoquímica multiplex e na imagem multiespectral, caracterizar a densidade e o agrupamento das principais células imunes simultaneamente no endométrio continua sendo um desafio. Este artigo descreve um protocolo detalhado de coloração multiplex e imagens para a localização simultânea de quatro tipos de células imunes no endométrio.

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Biologia Questão 174 Multiplex Fluorescência Células Imunes Métodos Coloração
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