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Immunology and Infection

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Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
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Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Article DOI: 10.3791/62937-v • 09:47 min • August 13th, 2021
August 13th, 2021 •
1, 1, 2, 1
1Department of Biology, San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

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Summary
Automatic Translation

Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.

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Selective Cleaning Wild Caenorhabditis Nematodes Intestinal Microbiome Bacteria Enrichment Identification Novel Microbe Host Microbe Interactions Non-culturable Pathogens NGM Plates OP51 Escherichia Coli Dauer Stage M9 Minimal Salts Media Centrifuge Tube
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