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Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
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Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Article DOI: 10.3791/62940-v • 08:30 min • July 12th, 2021
July 12th, 2021 •
1, 1, 2, 3, 1, 1, 1,3
1Department of Reproductive Endocrinology, Wagistrasse 14, University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18, University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology, University of Pittsburgh

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Summary
Automatic Translation

Crosstalk between mammary epithelial cells and endothelial cells importantly contributes to breast cancer progression, tumor growth, and metastasis. In this study, spheroids have been made from breast cancer cells together with vascular and/or lymphatic endothelial cells and demonstrate their applicability as an in vitro system for breast cancer research.

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Mammary Epithelial Cells Endothelial Cells Spheroids Functional In Vitro Model Breast Cancer Research Molecular Mechanisms Cancer Cell Endothelial Cell Interactions Proliferation Metastasis 3D Model Pathophysiology Treatment Of Breast Cancer Giovanna Azzarito PhD Student Treating Transfecting Plated Cells Hanks' Balanced Salt Solution HBSS Calcium And Magnesium HUVECs Green Dye Growth Medium Enzymatic Detachment Re-agent Trypsin Fetal Calf Serum Or FCS Medium
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