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Neuroscience

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JoVE Journal Neuroscience
Påvisning af G Protein-koblet receptor udtryk i mus vagal afferent neuroner ved hjælp af Multiplex In Situ Hybridization
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Påvisning af G Protein-koblet receptor udtryk i mus vagal afferent neuroner ved hjælp af Multiplex In Situ Hybridization

Article DOI: 10.3791/62945-v • 08:16 min • September 20th, 2021
September 20th, 2021 •
1, 1
1Center for Hypothalamic Research and Department of Internal Medicine, UTSouthwestern Medical Center at Dallas

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Multiplex in situ hybridization (ISH) blev ansat til samtidig at visualisere udskrifter for to G protein-koblede receptorer og en transskription faktor i hele vagal ganglionic kompleks af den voksne mus. Denne protokol kan bruges til at generere nøjagtige kort over de transskriptionelle profiler af vagale afferent neuroner.

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Neurovidenskab Neuroanatomi signalering histologi transskription transmembrane receptorer perifert nervesystem dissektion konfokal mikroskopi fluorescens
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