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Journal / Biochemistry / 生細胞におけるタンパク質相互作用とタンパク質ダイナミクスを研究するための二色蛍光相互相関分光法…
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Biochemistry
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JoVE Journal Biochemistry
生細胞におけるタンパク質相互作用とタンパク質ダイナミクスを研究するための二色蛍光相互相関分光法
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
Journal (Biochemistry)
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Journal (Biochemistry)
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
 
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生細胞におけるタンパク質相互作用とタンパク質ダイナミクスを研究するための二色蛍光相互相関分光法

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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我々は、現代の蛍光標識技術を用いて生細胞における膜受容体ダイナミクスを研究するために、フェルスター共鳴エネルギー伝達(FRET)と組み合わせた生細胞二重色蛍光相互相関分光(FCCS)を行う実験プロトコルとデータ分析ワークフローを提示する。

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生化学、178号、
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