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A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

Article DOI: 10.3791/62967-v • 09:47 min • August 26th, 2021
August 26th, 2021 •
1, 1, 2, 3, 3, 3, 4, 5, 6, 6, 4, 4, 7, 5, 3, 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute

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Summary
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This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.

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Algal Blooms Harmful Algal Blooms Chile Metabarcoding Analysis Standardized Procedure Monitoring Toxic Algal Species Early Warning Community Protection Microscopy Procedures Sample Collection Sample Pre-treatment Metabarcoding Analysis Water Sample Filtration 16S RRNA Analysis 18S RRNA Analysis DNA Extraction Chelex Method Microscopic Analysis
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