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Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica
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Una procedura standardizzata per il monitoraggio delle fioriture algali dannose in Cile mediante analisi metabarcodifica

Article DOI: 10.3791/62967-v • 09:47 min • August 26th, 2021
August 26th, 2021 •
1, 1, 2, 3, 3, 3, 4, 5, 6, 6, 4, 4, 7, 5, 3, 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute

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Questo protocollo introduce fasi di analisi metabarcodifica, mirate ai geni rRNA 16S e rRNA 18S, per monitorare le fioriture algali dannose e il microbioma associato nei campioni di acqua di mare. È un potente strumento basato su molecole, ma richiede diverse procedure, che sono spiegate visivamente qui passo dopo passo.

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Scienze ambientali Numero 174 Fioriture di alghe nocive Cile Monitoraggio HAB analisi metabarcoding mutualismo algale-batterico fitoplancton acquacoltura di salmone
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