The recruitment of leukocytes and platelets constitutes an essential component necessary for the effective growth of collateral arteries during arteriogenesis. Multiphoton microscopy is an efficient tool for tracking cell dynamics with high spatio-temporal resolution in vivo and less photo-toxicity to study leukocyte recruitment and extravasation during arteriogenesis.
Lasch, M., Vladymyrov, M., van den Heuvel, D., Götz, P., Deindl, E., Ishikawa-Ankerhold, H. Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model. J. Vis. Exp. (175), e62969, doi:10.3791/62969 (2021).