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Biochemistry

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JoVE Journal Biochemistry
Prøvepræparat og relativ kvantitet ved hjælp af reduktiv methylering af aminer til peptidomicsstudier
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Journal (Biochemistry)
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Journal (Biochemistry)
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
 
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Prøvepræparat og relativ kvantitet ved hjælp af reduktiv methylering af aminer til peptidomicsstudier

Article DOI: 10.3791/62971-v • 08:00 min • November 4th, 2021
November 4th, 2021 •
1,2, 1, 1, 1,2
1Department of Biological and Environmental Sciences, Bioscience Institute, Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute, Sao Paulo State University (UNESP)

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I denne artikel beskrives en prøveforberedelsesmetode baseret på varmeinaktivering for at bevare endogene peptider, så man undgår nedbrydning efter mortem, efterfulgt af relativ kvantitet ved hjælp af isotopmærkning plus LC-MS.

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