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JoVE Journal
Bioengineering
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使用Y-27632的原发性人类金吉瓦尔表皮细胞的隔离与培养
 
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使用Y-27632的原发性人类金吉瓦尔表皮细胞的隔离与培养

Article DOI: 10.3791/62978-v 06:47 min November 6th, 2021
November 6th, 2021

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Summary

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在这里,我们提出了一个改进的方法,通过添加岩石抑制剂,Y-27632,以传统的方法隔离和培养人类金吉瓦尔上皮细胞。这种方法更容易,更省时,提高干细胞的特性,并产生更多的高潜力上皮细胞,无论是为实验室和临床应用。

Tags

生物工程,第177期,人参上皮细胞,酶法,直接外生法,细胞分离,细胞培养,Y-27632,Rho相关激酶抑制剂,免疫荧光分析
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