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JoVE Journal Bioengineering
Isolation og kultur af primære menneskelige gingivale epitelceller ved hjælp af Y-27632
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Isolation og kultur af primære menneskelige gingivale epitelceller ved hjælp af Y-27632

Article DOI: 10.3791/62978-v • 06:47 min • November 6th, 2021
November 6th, 2021 •
1,2,3, 4, 3, 1, 1, 1, 1, 1, 1, 2,5, 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

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Her præsenterer vi en modificeret metode til isolering og kultur af menneskelige gingivale epitelceller ved at tilføje Rock-hæmmeren, Y-27632, til den traditionelle metode. Denne metode er lettere, mindre tidskrævende, forbedrer stamcelleegenskaberne og producerer et større antal højpotentialede epitelceller både til laboratoriet og til kliniske anvendelser.

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Bioengineering gingival epitelceller enzymatisk metode direkte explant metode celle isolation cellekultur Y-27632 Rho-associerede kinase hæmmer immunofluorescence analyse
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