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Biochemistry

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JoVE Journal Biochemistry
Micropatterning Transmission ElektronMikroskopiGitre til direkte cellepositionering i hele celle kryo-elektrontomografi arbejdsprocesser
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Micropatterning Transmission ElektronMikroskopiGitre til direkte cellepositionering i hele celle kryo-elektrontomografi arbejdsprocesser

Article DOI: 10.3791/62992-v • 09:53 min • September 13th, 2021
September 13th, 2021 •
*1,2,3, *1,4, 1,2,3, 1,2,3,5
1Department of Biochemistry, University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, 4Department of Chemistry, University of Wisconsin, Madison, 5Morgridge Institute for Research
* These authors contributed equally

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Målet med denne protokol er at lede celle vedhæftning og vækst til målrettede områder af net til kryo-elektronmikroskopi. Dette opnås ved at anvende et anti-fouling lag, der er ablated i bruger-specificerede mønstre efterfulgt af aflejring af ekstra cellulære matrix proteiner i de mønstrede områder før celle såning.

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Biokemi Udgave 175 cellekultur maskeløs fotopatterning mikromaling kryo-elektronmikroskopi kryo-EM kryo-elektrontomografi kryo-ET korrelativt lys og elektronmikroskopi CLEM fluorescenslysmikroskopi fLM
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