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Journal / Biochemistry / Micropatterning Transmissão Electron Microscopy…
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Biochemistry

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JoVE Journal Biochemistry
Micropatterning Transmissão Electron Microscopy Grids para Posicionamento Direto celular dentro de fluxos de trabalho de tomografia crio-elétron inteira
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Micropatterning Transmissão Electron Microscopy Grids para Posicionamento Direto celular dentro de fluxos de trabalho de tomografia crio-elétron inteira

Article DOI: 10.3791/62992-v • 09:53 min • September 13th, 2021
September 13th, 2021 •
*1,2,3, *1,4, 1,2,3, 1,2,3,5
1Department of Biochemistry, University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, 4Department of Chemistry, University of Wisconsin, Madison, 5Morgridge Institute for Research
* These authors contributed equally

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O objetivo deste protocolo é direcionar a adesão celular e o crescimento para áreas direcionadas de redes para microscopia crio-elétron. Isso é conseguido aplicando uma camada anti-incrustada que é ablada em padrões especificados pelo usuário seguido pela deposição de proteínas de matriz extracelular nas áreas padronizadas antes da semeadura celular.

Tags

Bioquímica Edição 175 cultura celular fotopatterning sem máscara micropatterning microscopia crio-elétron crio-EM tomografia crio-elétron crio-ET luz correlativa e microscopia eletrônica CLEM microscopia de luz fluorescência fLMM
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