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Automatisierung der Aggregatquantifizierung bei Caenorhabditis elegans
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Automatisierung der Aggregatquantifizierung bei Caenorhabditis elegans

Article DOI: 10.3791/62997-v • 07:50 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1,2, 1, 1
1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

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Das folgende Protokoll beschreibt die Entwicklung und Optimierung eines Hochdurchsatz-Workflows für die Wurmkultivierung, Fluoreszenzbildgebung und automatisierte Bildverarbeitung zur Quantifizierung von Polyglutaminaggregaten als Bewertung von Veränderungen der Proteostase.

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Biologie Ausgabe 176 C. elegans Proteostase Polyglutamin Aggregation
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