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Automatizando quantificação agregada em caenorhabditis elegans
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Automatizando quantificação agregada em caenorhabditis elegans

Article DOI: 10.3791/62997-v • 07:50 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1,2, 1, 1
1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

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O protocolo a seguir descreve o desenvolvimento e otimização de um fluxo de trabalho de alto rendimento para cultivo de vermes, imagens de fluorescência e processamento automatizado de imagens para quantificar agregados de poliglutamina como uma avaliação de mudanças na proteostase.

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Biologia Edição 176 C. elegans proteostase poliglutamina agregação
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