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Journal / Biochemistry / Produksjon av siRNA-lastede Lipid Nanopartikler…
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Biochemistry

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JoVE Journal Biochemistry
Produksjon av siRNA-lastede Lipid Nanopartikler ved hjelp av en mikrofluidisk enhet
Mouse Adipose Tissue Collection and Processing for RNA Analysis
Journal (Biochemistry)
Mouse Adipose Tissue Collection and Processing for RNA Analysis
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Journal (Biochemistry)
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
 
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Produksjon av siRNA-lastede Lipid Nanopartikler ved hjelp av en mikrofluidisk enhet

Article DOI: 10.3791/62999-v • 06:02 min • March 22nd, 2022
March 22nd, 2022 •
1,2, 3, 3, 3, 1, 1, 1
1Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering, Hokkaido University

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Mikrofluidbaserte lipid nanopartikkelproduksjonsmetoder (LNP) har vakt oppmerksomhet i legemiddelleveringssystemer (DDS), inkludert RNA-levering. Denne protokollen beskriver fabrikasjon, LNP (siRNA-lastet LNP) produksjon, og LNP evalueringsprosesser ved hjelp av vår opprinnelige mikrofluidiske enhet kalt iLiNP.

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