Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Madison Trujillo; Taylor McElroy; Taurean Brown; Pilar Simmons; Fabio Ntagwabira; Anti&#241;o R. Allen

doi:10.3791/63007

Journal

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Neuroscience

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Dissociation mécanique et enzymatique combinée du tissu hippocampique du cerveau de la souris

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Neuroscience

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JoVE Journal Neuroscience

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

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Dissociation mécanique et enzymatique combinée du tissu hippocampique du cerveau de la souris

3,333 Views

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07:14 min

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October 21, 2021

DOI:

10.3791/63007-v

DOI:

10.3791/63007-v

•

07:14 min

October 21, 2021

•

11 Views

Madison Trujillo^1,2,3, Taylor McElroy^1,2,3, Taurean Brown^1,2,3, Pilar Simmons^1,2, Fabio Ntagwabira^1,2,3, Antiño R. Allen^1,2,3

¹Division of Radiation Health,University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Summary

Ce protocole de dissociation des cellules neurales est destiné aux échantillons avec une faible quantité de matière première et donne une suspension unicellulaire hautement viable pour l’analyse en aval, avec des étapes de fixation et de coloration facultatives.

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Dissociation mécanique et enzymatique combinée du tissu hippocampique du cerveau de la souris

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Summary

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