Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Madison Trujillo; Taylor McElroy; Taurean Brown; Pilar Simmons; Fabio Ntagwabira; Anti&#241;o R. Allen

doi:10.3791/63007

Journal

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Neuroscience

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Disociación mecánica y enzimática combinada del tejido del hipocampo cerebral de ratón

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

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Disociación mecánica y enzimática combinada del tejido del hipocampo cerebral de ratón

3,333 Views

•

07:14 min

•

October 21, 2021

DOI:

10.3791/63007-v

DOI:

10.3791/63007-v

•

07:14 min

October 21, 2021

•

11 Views

Madison Trujillo^1,2,3, Taylor McElroy^1,2,3, Taurean Brown^1,2,3, Pilar Simmons^1,2, Fabio Ntagwabira^1,2,3, Antiño R. Allen^1,2,3

¹Division of Radiation Health,University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Summary

Este protocolo de disociación de células neuronales está destinado a muestras con una baja cantidad de material de partida y produce una suspensión unicelular altamente viable para el análisis posterior, con pasos opcionales de fijación y tinción.

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Disociación mecánica y enzimática combinada del tejido del hipocampo cerebral de ratón

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Summary

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