Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Madison Trujillo; Taylor McElroy; Taurean Brown; Pilar Simmons; Fabio Ntagwabira; Anti&#241;o R. Allen

doi:10.3791/63007

Journal

/

Neuroscience

/

Fare Beyni Hipokampal Dokusunun Kombine Mekanik ve Enzimatik Ayrışması

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Please note that all translations are automatically generated. Click here for the English version.

Fare Beyni Hipokampal Dokusunun Kombine Mekanik ve Enzimatik Ayrışması

3,333 Views

•

07:14 min

•

October 21, 2021

DOI:

10.3791/63007-v

DOI:

10.3791/63007-v

•

07:14 min

October 21, 2021

•

11 Views

Madison Trujillo^1,2,3, Taylor McElroy^1,2,3, Taurean Brown^1,2,3, Pilar Simmons^1,2, Fabio Ntagwabira^1,2,3, Antiño R. Allen^1,2,3

¹Division of Radiation Health,University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Summary

Bu nöral hücre ayrışma protokolü, düşük miktarda başlangıç malzemesine sahip numuneler için tasarlanmıştır ve isteğe bağlı sabitleme ve boyama adımlarıyla aşağı akış analizi için oldukça uygun bir tek hücreli süspansiyon sağlar.

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Fare Beyni Hipokampal Dokusunun Kombine Mekanik ve Enzimatik Ayrışması

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Summary

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