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Immunology and Infection

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JoVE Journal Immunology and Infection
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Article DOI: 10.3791/63028-v • 12:53 min • March 16th, 2022
March 16th, 2022 •
*1, *1, 1, 2, 2, 1, 1, 1, 1, 1, 2, 1,3
1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 2Department of Pathology and Laboratory Medicine, University of California, 3School of Medicine, Radiation Oncology, University of California Davis
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes using commercial, cell-free protein expression kits to produce membrane proteins supported in nanodisc that can be used as antigens in subunit vaccines.

Tags

Cell-Free Scaled Production Adjuvant Addition Recombinant Major Outer Membrane Protein Chlamydia Muridarum Vaccine Development Subunit Vaccine Antigens Membrane Proteins Protein Production Protein Purification Lipid Membrane Native-like Confirmation Formulation With Adjuvants Cell-free Expression Tagless Structure Scalable Process Commercial Companies Chlamydia Antigen Pathogen Vaccine Development Cancer Vaccine Development MOMP-tNLPs Reconstitution Buffer EDTA-free Protease Inhibitor
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