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JoVE Journal Neuroscience
Digitale Tropfen-PCR-Methode zur Quantifizierung der AAV-Transduktionseffizienz in der Murinen Netzhaut
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Digitale Tropfen-PCR-Methode zur Quantifizierung der AAV-Transduktionseffizienz in der Murinen Netzhaut

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Dieses Protokoll zeigt, wie die AAV-Transduktionseffizienz in der Netzhaut der Maus mittels digitaler Tröpfchen-PCR (dd-PCR) in Verbindung mit AAV-Produktion im kleinen Maßstab, intravitrealer Injektion, retinaler Bildgebung und retinaler genomischer DNA-Isolierung quantifiziert werden kann.

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Neuroscience Ausgabe 178
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