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JoVE Journal Neuroscience
Metodo Digital Droplet PCR per la quantificazione dell'efficienza di trasduzione AAV nella retina murina
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Metodo Digital Droplet PCR per la quantificazione dell'efficienza di trasduzione AAV nella retina murina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Questo protocollo presenta come quantificare l'efficienza di trasduzione AAV nella retina del topo utilizzando la PCR a goccia digitale (dd-PCR) insieme alla produzione di AAV su piccola scala, all'iniezione intravitreale, all'imaging retinico e all'isolamento del DNA genomico retinico.

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Neuroscienze Numero 178
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