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Journal / Developmental Biology / Rapid Isolation of Single Cells from Mouse and…
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Developmental Biology

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JoVE Journal Developmental Biology
Rapid Isolation of Single Cells from Mouse and Human Teeth
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Rapid Isolation of Single Cells from Mouse and Human Teeth

Article DOI: 10.3791/63043-v • 06:09 min • October 28th, 2021
October 28th, 2021 •
1, 1, 2, 2,3
1Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research, Medical University of Vienna, 3Department of Physiology and Pharmacology, Karolinska Institute

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Summary
Automatic Translation

The current protocol presents a fast, efficient, and gentle method for isolating single cells suitable for single-cell RNA-seq analysis from a continuously growing mouse incisor, mouse molar, and human teeth.

Tags

Single Cells Mouse Teeth Human Teeth Isolation Rapid Isolation Single-cell Suspension Omics Methodology Tissue Processing Tissue Dissociation Extracellular Matrix-rich Tissues Dental Pulps Manual Challenge Euthanized Mouse Mandible Facial Muscles Masseter Temporomandibular Joint
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