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JoVE Journal
Biology

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小鼠原发性肝正弦内皮细胞的分离与表征
 
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小鼠原发性肝正弦内皮细胞的分离与表征

Article DOI: 10.3791/63062-v 08:22 min December 16th, 2021
December 16th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这里,我们概述并演示了原代小鼠肝脏正弦内皮细胞(LSEC)分离方案。该方案基于肝胶原酶灌注、低速离心纯化非实质细胞和CD146磁珠选择。我们还使用流式细胞术和扫描电子显微镜对这些分离的LSECs进行表型和表征。

Tags

生物学,第178期,肝正弦内皮细胞(LSECs),胶原酶灌注,磁珠阳性选择,流式细胞术,免疫组织化学,扫描电子显微镜
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