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JoVE Journal Neuroscience
Imágenes en vivo del estado redox de glutatión mitocondrial en neuronas primarias utilizando un indicador ratiométrico
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Imágenes en vivo del estado redox de glutatión mitocondrial en neuronas primarias utilizando un indicador ratiométrico

Article DOI: 10.3791/63073-v • 07:47 min • October 20th, 2021
October 20th, 2021 •
1, 1, 2, 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine

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Este artículo describe un protocolo para determinar las diferencias en el estado redox basal y las respuestas redox a las perturbaciones agudas en las neuronas primarias del hipocampo y cortical utilizando microscopía viva confocal. El protocolo se puede aplicar a otros tipos de células y microscopios con modificaciones mínimas.

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Neurociencia Número 176 Grx1-roGFP2 microscopía confocal estrés oxidativo neuronas del hipocampo excitotoxicidad potencial de membrana mitocondrial
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