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Journal / Biochemistry / Uracil-DNA Glycosylase Assay door Matrix-assisted …
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Biochemistry

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JoVE Journal Biochemistry
Uracil-DNA Glycosylase Assay door Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
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Uracil-DNA Glycosylase Assay door Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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Een niet-gelabelde, niet-radio-isotopische methode om uracil-DNA glycosylase activiteit te testen werd ontwikkeld met behulp van MALDI-TOF massaspectrometrie voor directe apurine / apyrimidinische site-bevattende productanalyse. De test bleek vrij eenvoudig, specifiek, snel en gemakkelijk te gebruiken voor DNA-glycosylasemeting.

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Biochemie Nummer 182 Base excisie reparatie MALDI-TOF massaspectrometrie Uracil-DNA glycosylase apurine/apyrimidinische site glycosylaseremmer
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