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Journal / Biochemistry / 매트릭스 보조 레이저 탈착/이온화 비행 시간 질량 분광법 분석에 의한 우라실-DNA…
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Biochemistry

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JoVE Journal Biochemistry
매트릭스 보조 레이저 탈착/이온화 비행 시간 질량 분광법 분석에 의한 우라실-DNA 글리코실라제 분석
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Journal (Biochemistry)
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Journal (Biochemistry)
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
 
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매트릭스 보조 레이저 탈착/이온화 비행 시간 질량 분광법 분석에 의한 우라실-DNA 글리코실라제 분석

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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우라실-DNA 글리코실라제 활성을 분석하기 위한 비표지, 비-방사성-동위원소 방법은 직접 아푸린산/아피리미딘 부위 함유 생성물 분석을 위해 MALDI-TOF 질량 분광법을 사용하여 개발되었다. 이 분석은 DNA 글리코실라제 측정에 매우 간단하고, 구체적이며, 빠르고, 사용하기 쉬운 것으로 판명되었습니다.

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생화학 문제 182 염기 절제 복구 MALDI-TOF 질량 분광법 우라실-DNA 글리코실라제 아푸린산/아피리미딘 부위 글리코실라제 억제제
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