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Biochemistry

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JoVE Journal Biochemistry
Gerando organoides cardíacos humanos auto-montados derivados de células-tronco pluripotentes
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Journal (Biochemistry)
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Journal (Biochemistry)
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
 
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Gerando organoides cardíacos humanos auto-montados derivados de células-tronco pluripotentes

Article DOI: 10.3791/63097-v • 08:56 min • September 15th, 2021
September 15th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, 2Department of Biomedical Engineering, College of Engineering, Michigan State University

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Aqui, descrevemos um protocolo para criar organoides cardíacos humanos relevantes de desenvolvimento (hHOs) usando eficientemente células-tronco pluripotentes humanas por auto-organização. O protocolo conta com a ativação sequencial de pistas de desenvolvimento e produz tecidos cardíacos humanos altamente complexos e funcionalmente relevantes.

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Bioquímica Edição 175
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