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Journal / Neuroscience / Intracerebral transplantation og in vivo…
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Neuroscience

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JoVE Journal Neuroscience
Intracerebral transplantation og in vivo bioluminescens sporing af humane neurale stamceller i musehjernen
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Intracerebral transplantation og in vivo bioluminescens sporing af humane neurale stamceller i musehjernen

Article DOI: 10.3791/63102-v • 06:12 min • January 27th, 2022
January 27th, 2022 •
1,2, 1, 1, 1, 1, 1, 1, *1,2, *1,2
1Institute for Regenerative Medicine, University of Zurich, 2Neuroscience Center Zurich, University of Zurich and ETH Zurich
* These authors contributed equally

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Vi beskriver den intraparenkymale transplantation af humane neurale stamceller transduceret med en dobbelt reportervektor, der udtrykker luciferasegrønt fluorescerende protein (GFP) i musehjernen. Efter transplantation måles luciferasesignalet gentagne gange ved hjælp af in vivo-bioluminescens og GFP-ekspressionerende podede celler identificeret i hjernesektioner ved hjælp af fluorescensmikroskopi.

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Neurovidenskab Udgave 179 Celletransplantation luciferase in vivo-billeddannelse GFP hjernesektion intraparenkymtransplantation
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