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Neuroscience

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JoVE Journal Neuroscience
Transplante intracerebral e rastreamento de bioluminescência in vivo de células progenitoras neurais humanas no cérebro do camundongo
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Transplante intracerebral e rastreamento de bioluminescência in vivo de células progenitoras neurais humanas no cérebro do camundongo

Article DOI: 10.3791/63102-v • 06:12 min • January 27th, 2022
January 27th, 2022 •
1,2, 1, 1, 1, 1, 1, 1, *1,2, *1,2
1Institute for Regenerative Medicine, University of Zurich, 2Neuroscience Center Zurich, University of Zurich and ETH Zurich
* These authors contributed equally

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Descrevemos o transplante intraparenquimal de células progenitoras neurais transduzidas com um vetor de repórter duplo expressando proteína fluorescente verde-luciferase (GFP) no cérebro do camundongo. Após o transplante, o sinal de luciferase é repetidamente medido usando bioluminescência in vivo e células enxertadas expressas por GFP identificadas em seções cerebrais usando microscopia de fluorescência.

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Neurociência Edição 179 Transplante celular luciferase imagem in vivo GFP seção cerebral transplante intraparenquimal
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