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Journal / Neuroscience / Intracerebral transplantation och in vivo…
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Neuroscience

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JoVE Journal Neuroscience
Intracerebral transplantation och in vivo bioluminescens spårning av mänskliga neurala stamceller i mushjärnan
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Intracerebral transplantation och in vivo bioluminescens spårning av mänskliga neurala stamceller i mushjärnan

Article DOI: 10.3791/63102-v • 06:12 min • January 27th, 2022
January 27th, 2022 •
1,2, 1, 1, 1, 1, 1, 1, *1,2, *1,2
1Institute for Regenerative Medicine, University of Zurich, 2Neuroscience Center Zurich, University of Zurich and ETH Zurich
* These authors contributed equally

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Vi beskriver den intraparenkymala transplantationen av humana neurala stamceller transducerade med en dubbel reportervektor som uttrycker luciferasgrönt fluorescerande protein (GFP) i mushjärnan. Efter transplantation mäts luciferassignalen upprepade gånger med användning av in vivo bioluminescens och GFP-uttryckande ympade celler identifierade i hjärnsektioner med användning av fluorescensmikroskopi.

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Neurovetenskap utgåva 179 Celltransplantation luciferas in vivo-avbildning GFP hjärnsektion intraparenkymal transplantation
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