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JoVE Journal Neuroscience
Durchflusszytometrische Analyse multipler mitochondrialer Parameter in humaninduzierten pluripotenten Stammzellen und deren neuronalen und glialen Derivaten
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Durchflusszytometrische Analyse multipler mitochondrialer Parameter in humaninduzierten pluripotenten Stammzellen und deren neuronalen und glialen Derivaten

Article DOI: 10.3791/63116-v • 06:09 min • November 8th, 2021
November 8th, 2021 •
*1,2, *1,2,3,4, 1,2, 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
* These authors contributed equally

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Diese Studie berichtet über einen neuartigen Ansatz zur Messung mehrerer mitochondrialer Funktionsparameter basierend auf Durchflusszytometrie und Doppelfärbung mit zwei fluoreszierenden Reportern oder Antikörpern, um Veränderungen des mitochondrialen Volumens, des mitochondrialen Membranpotentials, des reaktiven Sauerstoffgehalts, der mitochondrialen Atmungskettenzusammensetzung und der mitochondrialen DNA zu erkennen.

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Neurowissenschaften Ausgabe 177
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