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JoVE Journal
Biochemistry

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细胞膜亲和色谱柱鉴定与固定化托肌球蛋白激酶受体B相互作用的特化植物代谢物
 
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细胞膜亲和色谱柱鉴定与固定化托肌球蛋白激酶受体B相互作用的特化植物代谢物

Article DOI: 10.3791/63118-v 11:44 min January 19th, 2022
January 19th, 2022

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Summary

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该协议描述了用含有功能性跨膜滋养肌球蛋白激酶受体B蛋白的固定化细胞膜片段制备细胞膜亲和色谱(CMAC)柱。还解释了使用CMAC色谱柱鉴定与这些受体相互作用并存在于复杂天然混合物中的特化植物代谢物。

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生物化学, 第179期,
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