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Journal / Biochemistry / Cellulær membran affinitet kromatografi kolonner…
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Biochemistry

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JoVE Journal Biochemistry
Cellulær membran affinitet kromatografi kolonner for å identifisere spesialiserte plante metabolitter samhandler med immobilisert tropomyosin kinase reseptor B
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Cellulær membran affinitet kromatografi kolonner for å identifisere spesialiserte plante metabolitter samhandler med immobilisert tropomyosin kinase reseptor B

Article DOI: 10.3791/63118-v • 11:44 min • January 19th, 2022
January 19th, 2022 •
1,2, 1, 1, 1, 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

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Protokollen beskriver fremstillingen av cellemembran affinitet kromatografi (CMAC) kolonner med immobiliserte cellemembranfragmenter som inneholder funksjonelle transmembrane tropomyosin kinase reseptor B-proteiner. Bruken av CMAC-kolonner i identifisering av spesialiserte plantemetabolitter som samhandler med disse reseptorene og tilstede i komplekse naturlige blandinger, forklares også.

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