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Bioengineering

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JoVE Journal Bioengineering
Analyse spatio-temporelle bidimensionnelle automatisée de sondes FRET mobiles à molécule unique
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Analyse spatio-temporelle bidimensionnelle automatisée de sondes FRET mobiles à molécule unique

Article DOI: 10.3791/63124-v • 08:26 min • November 23rd, 2021
November 23rd, 2021 •
1, 2, 2, 2, 1
1Institute of Applied Physics, TU Wien, 2Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna

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Cet article présente une méthode d’analyse spatio-temporelle de sondes mobiles à transfert d’énergie de résonance de Förster (smFRET) à molécule unique utilisant la microscopie à fluorescence à grand champ. La boîte à outils logicielle nouvellement développée permet de déterminer les traces temporelles smFRET des sondes en mouvement, y compris l’efficacité FRET correcte et les positions moléculaires, en tant que fonctions du temps.

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Bioingénierie numéro 177
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