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Bioengineering

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JoVE Journal Bioengineering
Automatiserad tvådimensionell Spatiotemporal analys av mobila enmolekyliga FRET-sonder
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Automatiserad tvådimensionell Spatiotemporal analys av mobila enmolekyliga FRET-sonder

Article DOI: 10.3791/63124-v • 08:26 min • November 23rd, 2021
November 23rd, 2021 •
1, 2, 2, 2, 1
1Institute of Applied Physics, TU Wien, 2Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna

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Denna artikel presenterar en metod för spatiotemporal analys av mobila, en-molekyl Förster resonans energiöverföring (smFRET)-baserade sonder med hjälp av widefield fluorescence mikroskopi. Den nyutvecklade mjukvaruverktygssatsen gör det möjligt att bestämning av smFRET-tidsspår av rörliga sonder, inklusive rätt FRET-effektivitet och de molekylära positionerna, som tidens funktioner.

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Bioengineering nummer 177
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